cellular markers lymphocyte subsets  (Santa Cruz Biotechnology)

Journal: Veterinary immunology and immunopathology

Article Title: Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus.

doi: 10.1016/j.vetimm.2012.06.013

Figure Lengend Snippet: Fig. 1. Representative forward scatter and side scatter gating of PBMC used to identify lymphocytes and their subsets by flow cytometry. Peripheral blood was isolated from cattle on day 0 (pre-infection) and at days 10 (acute phase) and 19 (recovery phase) following experimental JDV infection and PBMC isolated by Ficoll-Paque density separation. Following lymphocyte subset labelling (see following figures C5), cell suspensions were analysed by flow cytometry

Article Snippet: Antibodies and cellular markers Lymphocyte subsets were labelled with 2.5 g/ml ouse anti-bovine CD4 mAb (Serotec, UK), 5 g/ml mouse nti-bovine CD8 mAb (Serotec, UK) or 20 g/ml mouse nti-bovine CD21 mAb (Santa Cruz, USA) as a B-cell marker. n Alexa Fluor 488 (AF488) conjugated goat anti-mouse ross-absorbed secondary antibody (Invitrogen, Australia) as used to detect all reactive mAb antibodies (Table 1).

Techniques: Cytometry, Isolation, Infection

Journal: Veterinary immunology and immunopathology

Article Title: Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus.

doi: 10.1016/j.vetimm.2012.06.013

Figure Lengend Snippet: Fig. 5. Lymphocyte subset changes related to the febrile response fol- lowing

Article Snippet: Antibodies and cellular markers Lymphocyte subsets were labelled with 2.5 g/ml ouse anti-bovine CD4 mAb (Serotec, UK), 5 g/ml mouse nti-bovine CD8 mAb (Serotec, UK) or 20 g/ml mouse nti-bovine CD21 mAb (Santa Cruz, USA) as a B-cell marker. n Alexa Fluor 488 (AF488) conjugated goat anti-mouse ross-absorbed secondary antibody (Invitrogen, Australia) as used to detect all reactive mAb antibodies (Table 1).

Techniques: